A Method for Determining Total Carbon Dioxide in Small Volumes of Liquid

Autor:	Gary Ruston, Colin Little
Rok vydání:	1970
Předmět:	chemistry.chemical_compound chemistry Physiology Insect Science Diffusion Carbon dioxide Inorganic chemistry Animal Science and Zoology Aquatic Science Molecular Biology Ecology Evolution Behavior and Systematics Water vapor Barium hydroxide
Zdroj:	Journal of Experimental Biology. 52:395-400
ISSN:	1477-9145 0022-0949
DOI:	10.1242/jeb.52.2.395
Popis:	A microdiffusion method for estimating total dissolved carbon dioxide in volumes of approximately 1·5 μl. is described. The principle of the method is to displace the CO2 with acid, to absorb it in barium hydroxide solution and to measure the change ;n the freezing-point depression of this solution. The range of measurement is 0–30 mm/1. HCO3− with a standard deviation of ± 0·3–0·4 mm/1. In its present form the method cannot be used with smaller volumes without correcting for errors arising from the diffusion of water vapour between the sample and the barium hydroxide solution.
Databáze:	OpenAIRE
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