Comprehensive examination of somatic oncogene mutation in normal and pathologic thyroid tissues

Autor:	János Horányi, Gábor Speer, Bálint Tobiás, P. János Kósa, Zsolt Nagy, Bernadett Balla, Peter L. Lakatos, Balázs Járay, Eszter Székely, Eszter Bölöny, Roland Istók, István Takács, Csaba Halászlaki
Rok vydání:	2011
Předmět:	Neuroblastoma RAS viral oncogene homolog Mutation endocrine system diseases business.industry Thyroid General Medicine medicine.disease_cause medicine.disease Malignant transformation medicine.anatomical_structure Cancer research medicine KRAS HRAS PAX8 business Thyroid cancer
Zdroj:	Orvosi Hetilap. 152:672-677
ISSN:	1788-6120 0030-6002
Popis:	It is established that numerous somatic oncogene mutation (BRAF, NRAS, HRAS, KRAS) and gene translocations (RET/PTC, PAX8/PPAR-gamma) are associated with the development of thyroid cancer. In this study 22 intraoperative thyroid tissue samples (11 pathologic and 11 normal) were examined. Somatic single nucleotide polymorphisms were analyzed by LigthCycler melting method, while translocations were identified by real-time polymerase chain reaction technique. In tumorous sample 3 BRAF, 2 NRAS and one HRAS mutations were found, as well as one RET/PTC1 translocation. Results confirm international data showing that these oncogene mutations and translocations are linked to thyroid cancer. Cytological examination completed with genetic data may support the diagnosis of thyroid malignancies. In addition, genetic alterations may indicate malignant transformation and may become prognostic factors in future. Orv. Hetil., 2011, 152, 672–677.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::ab5c1ab574c27f1a719ab34ae875ab78 https://doi.org/10.1556/oh.2011.29097 Zobrazit plný text záznamu