Arginylation in a Partially Purified Fraction of 150k × g Supernatants of Axoplasm and Injured Vertebrate Nerves

Autor: Nicholas A. Ingoglia
Rok vydání: 2015
Předmět:
Zdroj: Methods in Molecular Biology ISBN: 9781493929344
DOI: 10.1007/978-1-4939-2935-1_4
Popis: Transfer RNA-mediated posttranslational protein modification by arginine has been demonstrated in vitro in axoplasm extruded from the giant axons of squid and in injured and regenerating vertebrate nerves. In nerve and axoplasm, the highest activity is found in a fraction of a 150,000 × g supernatant containing high molecular weight protein/RNA complexes but lacking molecules of
Databáze: OpenAIRE