Development of a Unique Rapid Test to Detect Anti-bodies Directed Against an Extended RBD of SARS-CoV-2 Spike Protein

Autor:	Patrick Romann, Larissa Brosi, Marie-Eve Überschlag, Michael Gerspach, Christian B. Gerhold, Anna Melone, Frank I Bantleon, Anna Weston, Daniele Dolce, Sherin Panikulam, Cedric Giegelmann, Thomas K. Villiger, Eric Kübler, Lorin Dirscherl
Rok vydání:	2021
Předmět:	Vaccination Immune system biology Antigen Immunity Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) biology.protein Spike Protein General Medicine General Chemistry Antibody Virology Serology
Zdroj:	CHIMIA. 75:446
ISSN:	2673-2424 0009-4293
Popis:	Serological testing for antibodies directed against SARS-CoV-2 in patients may serve as a diagnostic tool to verify a previous infection and as surrogate for an elicited humoral immune response, ideally conferring immunity after infection or vaccination. Here, we present the recombinant expression of an extended receptor binding domain (RBD) of the SARS-CoV-2 Spike protein used as capture antigen in a unique rapid immunoassay to detect the presence of RBD binding antibodies with high sensitivity and specificity. As currently available vaccines focus on the Spike RBD as target, the developed test can also be used to monitor a successful immune response after vaccination with an RBD based vaccine.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::ab405463611ed0ca04115d9e7bdbd779 https://doi.org/10.2533/chimia.2021.446 Zobrazit plný text záznamu