The effects of synthetic protease inhibitors on human proinsulin production by recombinantBacillus subtilis strain
| Autor: | Sterkin Viktor E, D. G. Popov, E. V. Parfenova, A. A. Novikov, A.Ya. Strongin |
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| Rok vydání: | 1990 |
| Předmět: |
Protease
Bacillaceae biology medicine.medical_treatment Prohormone Bioengineering General Medicine Bacillus subtilis biology.organism_classification Applied Microbiology and Biotechnology Bacillales Microbiology law.invention Biochemistry law medicine Recombinant DNA Bacteria Biotechnology Proinsulin medicine.drug |
| Zdroj: | Biotechnology Letters. 12:547-550 |
| ISSN: | 1573-6776 0141-5492 |
| DOI: | 10.1007/bf01030750 |
| Popis: | Two inexpensive inhibitors non-toxic to B. subtilis cells, O,O-diethyl-1-(N-α-hydrohexafluoro-isobutyryl)amino-1-methyl-propylphosphonate and O,O-diisobutyl-1-[2-(ethoxycarbonyl)amino-perfluoroprop-2-yl]-1-methylpropylphosphonate, when added to the culture broth, are able to prevent the proteolytic degradation of recombinant human proinsulin, secreted byB. subtilis AJ73apr73 npr− cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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