| Popis: |
The goal was the selection of infectious strains of microorganisms for the formation of experimental acute and chronic osteomyelitis. For this purpose, Staphylococcus aureus and Pseudomonas aeruginosa were selected. They were introduced 2 times in the amount of 6x109 mt/ml in each laboratory animal. Microbiological, clinical, and morphological signs of experimental acute and chronic osteomyelitis were observed in the pathological focus. To increase infection, both strains were seeded separately in nutrient broth and standardized. When introducing cultures of microorganisms into white, outbred mice, the need for immediate administration of an infected agent was taken into account. They were placed in cages (up to 20 mice were in one cage) at a height of 30–70 cm above the floor. All animals were quarantined 10 days after they were brought for a scientific experiment, and it was carried out after they were convinced that they did not have any infectious or other diseases. The cells that contained the experimental animals were cleaned and treated every morning in accordance with generally accepted rules. |
