Preliminary Studies on Cryopreservation of Vegetative Thalli of Some Economically Important Seaweeds of India

Autor:	Alkesh Dwivedi, Geetanjali Deshmukhe, P. L. Lalrinsanga, Sandip Chakraborty, Niraj Kumar, Nimai Barman
Rok vydání:	2009
Předmět:	food.ingredient Ecology biology Cryoprotectant Hypnea Aquatic Science Corticata biology.organism_classification Cryopreservation Thallus Horticulture food Lobata Aquatic plant Botany Gracilaria
Zdroj:	Journal of Applied Aquaculture. 21:250-262
ISSN:	1545-0805 1045-4438
DOI:	10.1080/10454430903317096
Popis:	The effects of cryoprotectants and their concentrations, prefreezing temperature, equilibration time, thawing temperature, and the regenerative efficacy of cryopreserved vegetative thalli (apical tips) of Gracilaria corticata J. Agardh., Ulva lobata Duetzing, and Hypnea musiformis (Wulfar) Lamouroux were evaluated. The apical tips were suspended in various cryoprotective solutions and slowly cooled to −40˚C over a period of 4 h. After the slow cooling step, the suspensions were immediately immersed in liquid nitrogen (LN), which were thawed after 2 days. Both U. lobata and H. musiformis survived maximally in 10% DMSO, whereas 10% glycerol was found most suitable for G. corticata. A slow cooling temperature of −40˚C and thawing temperature of 40˚C were found most suitable irrespective of the species. The equilibration time of 60 min was found most suitable for G. corticata, while 45 min was sufficient for both H. musiformi and U. lobata. Cryopreserved vegetative thalli were then successfully regenerated, t...
Databáze:	OpenAIRE
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