| Popis: |
Mammary involution after lactation is associated with extensive loss of secretory epithelial cells. Laddering of genomic DNA (Wyllie et al., 1980) and altered gene expression indicate that this cell loss occurs by apoptosis. Neither the mechanism nor the regulation of mammary cell death is well-characterised, but both circulating mammogenic hormones and locally-active factors may be involved. Apoptosis induced in lactating mouse mammary gland by litter removal was prevented by prolactin treatment (Sheffield and Kotolski, 1992), whereas non-milking of one gland of lactating goats increased DNA laddering in that gland (Quarrie et al., 1994). In this study, we present evidence for local induction of apoptosis in glands of lactating mice after 24 h of milk stasis. |
