Lipid Composition of Intact and Functional Mitochondria Isolated From Arabidopsis Thaliana Suspension Cultures

Autor: Jacques Davy de Virville, François Moreau, Thérèse Guillot-Salomon, Marie-Françoise Alin, Catherine Cantrel, Ivone Aaron
Rok vydání: 1995
Předmět:
Zdroj: Plant Lipid Metabolism ISBN: 9789048144983
DOI: 10.1007/978-94-015-8394-7_53
Popis: The crucifer Arabidopsis thaliana has become the most widely used model for plant molecular biology [1]. Mutants with alterations in chloroplast lipid composition have been isolated and characterized providing considerable informations about the pathways of lipid synthesis in plastid membranes [2]. By contrast, little is known about lipid biogenesis in plant mitochondria. These organelles, like plastids, are semi-autonomous with regard to the formation of their membrane lipids [3] . The isolation of mitochondria could be very useful to investigate the extent to which mitochondrial membranes import lipids from the endoplasmic reticulum. In addition, these mutants should prove very useful to study the consequences of lipid alteration on functional properties of these organelles. Here we report on the lipid analysis of mitochondria isolated from cell suspension cultures of Arabidopsis thaliana [4]. This work is a part of a program aimed at developing methods to fractionate Arabidopsis tissues.
Databáze: OpenAIRE