Genetic association study of C5178A and G10398A mitochondrial DNA variants with type 2 diabetes in Bangladeshi population
| Autor: | A. K. M. Mahbub Hasan, Md. Sohrab Alam, A.H.M. Nurun Nabi, Tahirah Yasmin, Sajoy Kanti Saha, Md. Ismail Hosen, Jobaida Akther, Nafiul Huda |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Genetics Mitochondrial DNA education.field_of_study endocrine system diseases biology Population NADH dehydrogenase nutritional and metabolic diseases DNA sequencing Minor allele frequency 03 medical and health sciences Restriction enzyme 030104 developmental biology 0302 clinical medicine 030220 oncology & carcinogenesis biology.protein Allele education Genetics (clinical) Genetic association |
| Zdroj: | Meta Gene. 19:23-31 |
| ISSN: | 2214-5400 |
| DOI: | 10.1016/j.mgene.2018.10.004 |
| Popis: | The mitochondrial DNA (mtDNA) G10398A variation (Ala→Thr) within the NADH dehydrogenase (ND3) subunit of complex I of the electron transport chain and C5178A within NADH dehydrogenase subunit (ND1-237, Leu → Met), have emerged as variations of clinical significance in disorders like type 2 diabetes (T2D). This study aims to explore the relationship of mtDNA C5178A and G10398A variations with T2D in Bangladeshi population. A total of 249 unrelated Bangladeshi populations (127 T2D and 122 healthy controls) were enrolled in this study. Specific DNA sequences within mitochondria were amplified by PCR followed by digestion at the polymorphic sites using AluI and DdeI restriction enzymes. Analyses revealed that 66.93% T2D and 59.02% healthy individuals carried the major allele ‘G’ at 10398 position of the mtGenome; while the minor allele ‘A’ was present in 33.07% T2D and 40.98% healthy individuals. G10398A polymorphism had no association with the T2D when total participants were considered. However, in male study participants, G10398A polymorphism can potentially be a protective marker for T2D (OR = 0.30, 95% CI: 0.13–0.67, p |
| Databáze: | OpenAIRE |
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