SYNTHESIS AND CHARACTERIZATION OF NEW N-TERMINAL BCHAIN MODIFIED HUMAN INSULIN ANALOGUES

Autor:	Jewad K. Shneine
Rok vydání:	2010
Předmět:	chemistry.chemical_classification Chromatography Dipeptide Edman degradation Stereochemistry Insulin medicine.medical_treatment Size-exclusion chromatography Ion chromatography Amino acid chemistry.chemical_compound chemistry medicine Asparagine Threonine
Zdroj:	Journal of Al-Nahrain University Science. 13:23-30
ISSN:	2519-0881 1814-5922
DOI:	10.22401/jnus.13.4.03
Popis:	New human insulin analogues were synthesized by fragment condensation reactions of N A1 , N B29 Msc protected native human insulin of different B-chain lengths with the dipeptide Boc-AsnThr-OH. Edman degradation was used to obtain shortened B-chain insulin intermediates. Purification of the products was achieved using gel filtration, ion exchange chromatography and RP-MPLC chromatography. Analysis of synthesized analogues accomplished by RP-HPLC chromatography and amino acid analysis. The modified insulin analogues with the amino acids Asparagine in the first position and Threonine in the second position of the N-terminal B-chain of insulin would be expected to show an enhanced T R* transition compared with that of wild-type structure.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::aabbf6f5a606e7c6f27a8e1c17e856d2 https://doi.org/10.22401/jnus.13.4.03 Zobrazit plný text záznamu