Creation of blast disease-resistant rice varieties with modern DNA-markers
Autor: | Elena Dubina, I. B. Nikitina, N. N. Korkina, E. S. Kharchenko, V. N. Shilovskiy, Zh. M. Mukhina, E. P. Maximenko, E. M. Kharitonov, L. V. Esaulova |
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Rok vydání: | 2015 |
Předmět: | |
Zdroj: | Russian Journal of Genetics. 51:752-756 |
ISSN: | 1608-3369 1022-7954 |
Popis: | Based on modern technologies of molecular DNA-markers, blast disease-resistance genes (Pi-ta, Pi-b, Pi-1, Pi-2, and Pi-33) were introgressed and pyramided into domestic rice varieties to give them long-term disease resistance. For that purpose, this case study uses SSR-markers closely linked to these genes, as well as intragenic markers of genes Pi-ta and Pi-b. Multiplex PCR systems were created for simultaneous identification of two resistance genes in the hybrid progeny for the following combinations: Pi-1 + Pi-2, Pi-ta + Pi-b, Pi-ta + Pi-33. |
Databáze: | OpenAIRE |
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