Tandem mass spectrometry linked fraction collection for the isolation of drug metabolites from biological matrices

Autor:	B. C. Sweatman, I. M. Ismail, Gordon J. Dear, R. S. Plumb, J. Ayrton
Rok vydání:	1999
Předmět:	Chromatography Protein mass spectrometry Chemistry Organic Chemistry Fraction (chemistry) Fractionation Tandem mass tag Top-down proteomics Tandem mass spectrometry Spectroscopy Sample preparation in mass spectrometry Drug metabolism Analytical Chemistry
Zdroj:	Rapid Communications in Mass Spectrometry. 13:886-894
ISSN:	1097-0231 0951-4198
DOI:	10.1002/(sici)1097-0231(19990530)13:10<886::aid-rcm577>3.0.co;2-b
Popis:	An automated mass spectrometric linked fraction collection system is described which enables on-line examination of isolated components based on molecular-ion and product-ion information. This system has been used successfully to specifically isolate drug-related material from complex biological fluids to aid structural identification. Metabolites have been isolated with sufficient purity to allow unequivocal characterisation by 1H nuclear magnetic resonance spectroscopy. This system has the advantage that isolation of the components of interest is not triggered by a simple contact closure. Therefore fraction collection is not biased by limitations in either the detector (e.g. insufficient sensitivity) or the analyst (e.g. programmed collection of predicted metabolites only). Furthermore, all isolated components are readily available post fractionation for additional screening.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::aab1cdbfc6954761385f17d7c401290f https://doi.org/10.1002/(sici)1097-0231(19990530)13:10<886::aid-rcm577>3.0.co Zobrazit plný text záznamu Plný text