Fabrication of benzylisothiocynate encapsulated nanoemulsion through ultrasonication: Augmentation of anticancer and antimicrobial attributes
| Autor: | S.K. Mehta, Raj Kumar, Rajendra Kumar, Shivani Uppal, Khushwinder Kaur, Satish Kumar Pandey |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Antifungal
High energy Chromatography Fabrication medicine.drug_class Chemistry Sonication 04 agricultural and veterinary sciences 02 engineering and technology 021001 nanoscience & nanotechnology Condensed Matter Physics Antimicrobial 040401 food science Atomic and Molecular Physics and Optics Electronic Optical and Magnetic Materials 0404 agricultural biotechnology Pulmonary surfactant Materials Chemistry medicine Microemulsion Physical and Theoretical Chemistry 0210 nano-technology Spectroscopy Water in oil |
| Zdroj: | Journal of Molecular Liquids. 263:324-333 |
| ISSN: | 0167-7322 |
| Popis: | The present work has been conceded with an observation to support the inherent advantages of nanoemulsions over microemulsions. This simply involves comparison of the difference in the synthesis of two formulations. Firstly, lemon oil/miglyl 812, oil in water and water in oil microemulsions were synthesized using tween 80 as a stabilizer. Secondly, the same formulation was used for the preparation of nanoemulsions. However, low concentration of Tween 80 failed to stabilize the nanoemulsion with simple stirring, shaking etc. Therefore, non toxic surfactant decyl-β- d -glucopyranoside was incorporated in the nanoformulation. Further, high energy ultrasound was utilized in the fabrication of nanoemulsion. The average size of nanoemulsion and microemulsion was found to be 31.68 ± 1.78 nm and 20.98 ± 2.88 nm, respectively. After the successful comparison of the systems, nanoemulsion was chosen for further study for encapsulation of Benzylisothiocynate. The system was characterized at pH (2−12) and various salt concentrations (0–500 mM) for 60 days. Time kill kinetics revealed that benzylisothiocynate nanoemulsion has stronger antibacterial/antifungal activity as compared to pure nanoemulsion. The cell viability of developed formulation tested on MDA-MB 231 cell lines have been found to be non toxic below concentration 4 μg/ml. |
| Databáze: | OpenAIRE |
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