Single walled carbon nanotubes induce cytotoxicity and oxidative stress in HEK293 cells
| Autor: | V. Himabindu, A. Rama Narsimha Reddy, Devarakonda R. Krishna, Y. Narsimha Reddy |
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| Rok vydání: | 2014 |
| Předmět: |
Health
Toxicology and Mutagenesis HEK 293 cells Glutathione medicine.disease_cause Pollution Cell membrane Lipid peroxidation chemistry.chemical_compound medicine.anatomical_structure chemistry Biochemistry Lactate dehydrogenase medicine Biophysics Environmental Chemistry Viability assay Cytotoxicity Oxidative stress |
| Zdroj: | Toxicological & Environmental Chemistry. 96:931-940 |
| ISSN: | 1029-0486 0277-2248 |
| DOI: | 10.1080/02772248.2014.993112 |
| Popis: | The aim of the present study was to evaluate the potential toxicity and general mechanisms involved in single walled carbon nanotubes (SWCNTs)-induced cytotoxicity using human embryonic kidney cell line (HEK293) cells. Carbon nanotubes (coded as CNT) used in this study were synthesized by the chemical vapor deposition method. To elucidate the possible mechanisms underlying SWCNT-induced cytotoxicity, cell viability, cell membrane damage (lactate dehydrogenase activity (LDH) assay), reduced glutathione (GSH), interleukin-8 (IL-8) and lipid peroxidation products levels were quantitatively assessed following SWCNT exposure for 48 hr using HEK293 cells. Exposure of cells to SWCNT at 3–300 μg/ml produced significant reduction in cell viability in a concentration-dependent manner. The IC50 value of SWCNT was found to be 87.58 μg/ml. Exposure of HEK cells to SWCNT at 10–100 μg/ml resulted in concentration-dependent cell membrane damage, increased production of IL-8, elevated levels of thiobarbituric acid reactiv... |
| Databáze: | OpenAIRE |
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