A simple and reliable preparative high-performance liquid chromatographic technique for isolation of a bioactive flavone diglycoside from and extract ofCuminum cyminumseeds

Autor:	Naresh Kumar Satti, Satya Narayan Sharma, Shubham Sharma, Imran Khan, Musarat Amina, K. A. Suri, Vishal Sharma, B. D. Gupta, Punita Sharma, R.K. Johri, Prabhu Dutt
Rok vydání:	2009
Předmět:	chemistry.chemical_classification Cuminum Chromatography chemistry biology Plant composition General Chemistry Carbon-13 NMR Mass spectrometry biology.organism_classification Flavones Quantitative analysis (chemistry) High-performance liquid chromatography
Zdroj:	Acta Chromatographica. 21:499-512
ISSN:	2083-5736 1233-2356
DOI:	10.1556/achrom.21.2009.3.12
Popis:	Summary In this paper we describe a sensitive and reproducible reversed-phase high-performance liquid chromatography (HPLC) method with photodiode-array detection for isolation and quantification of the bioactive hydrophilic constituent 7-(1-O-β-d-galacturonide-4′-(1-O-β-d-glucopyranosyl)-3′,4′,5,7-tetrahydroxyflavone, 1, from the seeds of Cuminum cyminum. Compound 1 was separated isocratically on a C18 preparative column, in high purity, after removal of solvents. The purity and identity of the compound were established by use of LC-mass spectrometry and by spectroscopic techniques (1H and 13C NMR). The purity of 1 was also confirmed by HPTLC.
Databáze:	OpenAIRE
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