Quantification of Porphyromonas gingivalis in chronic periodontitis patients associated with diabetes mellitus using real-time polymerase chain reaction
| Autor: | Soumya Makarla, Radhika M Bavle, Gadavalli Vera Venkata Satyakiran, M Sudhakara, G V Padmalatha, K Paremala |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Dentistry Gastroenterology Pathology and Forensic Medicine 03 medical and health sciences 0302 clinical medicine Diabetes mellitus Internal medicine medicine Tooth loss General Dentistry Porphyromonas gingivalis Periodontitis biology business.industry 030206 dentistry Periodontium medicine.disease biology.organism_classification Chronic periodontitis 030104 developmental biology Real-time polymerase chain reaction Otorhinolaryngology Etiology medicine.symptom business |
| Zdroj: | Journal of Oral and Maxillofacial Pathology. 20:413 |
| ISSN: | 0973-029X |
| DOI: | 10.4103/0973-029x.190933 |
| Popis: | Introduction: Periodontal diseases, if left untreated, can lead to tooth loss and affect at least one tooth in 80% of adults worldwide, with the main cause being a bacterial plaque. Among subgingival plaque bacterial species, Porphyromonas gingivalis has been implicated as a major etiological agent causing tooth loss. Diabetics and smokers are two patient groups at high risk for periodontal disease. The increase in the number of this organism with the coexistence of other pathogenic microbes leads to rapid destruction of the periodontium, premature loss of teeth and also because of its virulence has implications in systemic pathology. Our aim was to observe the involvement of P. gingivalis in diabetes mellitus (DM) patients associated with periodontitis with and without tobacco-associated habits and to compare them with periodontitis patients having no other systemic pathologies. Materials and Methods: Subgingival plaque samples from a total of seventy subjects were included in the study. DNA was isolated from the collected sample and was quantified using spectrophotometer for standardizing the polymerase chain reaction. The quantity of the isolated DNA was checked in a ultraviolet-visible spectrophotomer. Statistics: One-way ANOVA and Tukey's multiple post hoc procedures were carried out. Results: The maximum score of P. gingivalis was seen in periodontitis patients having DM, whereas the least score was seen in periodontitis patients having DM with tobacco smoking habit compared to the other groups. Conclusion: P. gingivalis count is significantly reduced in periodontitis patients having DM with smoking habit; it is concluded that P. gingivalis might not be a key causative organism responsible for the periodontal destruction in case of smokers despite the DM condition. The decrease in counts may be attributed to change in the local environment like chemical (tobacco nitrosamines) and physical changes preventing the growth of P. gingivalis. |
| Databáze: | OpenAIRE |
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