Optimization of Proliferating Cell Nuclear Antigen (PCNA) Immunohistochemical Staining: A Comparison of Methods Using Three Commercial Antibodies, Various Fixation Times, and Antigen Retrieval Solution

Autor:	Kenneth E. Gould, Natalie D. Macon, George E. Sandusky, Karen Mintze
Rok vydání:	1995
Předmět:	Histology Ileum Biology Molecular biology Staining Proliferating cell nuclear antigen Medical Laboratory Technology chemistry.chemical_compound medicine.anatomical_structure Antigen retrieval chemistry Antigen biology.protein medicine Immunohistochemistry Anatomy Antibody Fixation (histology)
Zdroj:	Journal of Histotechnology. 18:25-30
ISSN:	2046-0236 0147-8885
Popis:	The purpose of this study was to identify the optimal staining for cell proliferation in paraffin embedded sections of rabbit and pig ileum. Methods comparing zinc formalin or 10 % buffered formalin, 3 PCNA antibodies (19A2, PC10, MAB424), fixation time intervals at 4, 8,24, and 48 hr, and antigen retrieval solution were developed. Staining of nuclei in rabbit ileum was optimal after 4–8 hr in zinc formalin with the PC10, 19A2, and MAB424 antibodies, without antigen retrieval. All 3 antibodies produced optimal staining of nuclei in pig ileum fixed in zinc formalin 4–8 hr without antigen retrieval.After 4 hr fixation in 10% neutral buffered formalin (NBF), nuclei in both pig and rabbit were lightly stained in comparison to those fixed in zinc formalin. Minimal or no staining was seen at 8, 24, and 48 hr fixation in 10% NBF for rabbit ileum. Weak staining was seen at 28 and 48 hr in 10% NBF for pig ileal nuclei.Antigen retrieval methods did enhance nuclear staining with zinc formalin fixation and 10...
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::aa8ba0ee1cfa168e4e1be85b80724d41 https://doi.org/10.1179/his.1995.18.1.25 Zobrazit plný text záznamu