Hemolytic Plaque Formation to Allogeneic Human Type A and B Red Cells1

Autor:	Mutsuo Sasaki, Paul I. Terasaki
Rok vydání:	2008
Předmět:	Red Cell business.industry Pokeweed mitogen Immunology Stimulation General Medicine Human type Biochemistry Molecular biology In vitro Peripheral blood medicine.anatomical_structure Antigen Genetics medicine Immunology and Allergy business Sensitization
Zdroj:	Tissue Antigens. 13:189-194
ISSN:	1399-0039 0001-2815
DOI:	10.1111/j.1399-0039.1979.tb00783.x
Popis:	Plaque-forming cells can be generated from peripheral blood lymphocytes provided that the lymphocytes are prestimulated by in vitro sensitization in the presence of pokeweed mitogen. Of 91 blood type O persons whose lymphocytes were stimulated with red cell A antigen, 52% responded with plaque formation, whereas of 40 type O persons whose lymphocytes were stimulated with red cell B antigen, only 20% produced plaques. Similarly, reciprocal stimulation of type A and B persons showed higher plaque formation (65%) when 23 type B persons were stimulated with A than when 48 type A persons were stimulated with B red cells (19%). An optimal dilution of sensitizing cells is necessary and maximum time of sensitization was approximately 7 days.
Databáze:	OpenAIRE
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