| Autor: |
Zhi Fang Pan, Chun E Ren, Wei Guo Feng |
| Rok vydání: |
2014 |
| Předmět: |
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| Zdroj: |
Advanced Materials Research. :544-547 |
| ISSN: |
1662-8985 |
| DOI: |
10.4028/www.scientific.net/amr.884-885.544 |
| Popis: |
In this study, we succeed to establish the well in the well (WIW) culture system. Using this system we studied the effect of floating granulosa cells density on oocyte developmental competence. Results: Apoptosis rate of the floating cells were high (73.576.3%) and did not differ (P > 0.05) among follicles of different sizes. Hatching rates of blastocysts did not differ (P > 0.05) between oocytes cultured in the WIW system (13.3%) and those cultured in the group system (16.3%). In 4-8mm follicles, oocyte developmental competence is higher (P < 0.05) in follicles with 1030×105/mL floating cells than in follicles with < 10 or >30%×105/mL floating cells. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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