Akkurate Detektion seltener Gene und flexible Auswahl der Referenzgene
Autor: | Thorsten Traeger, Andreas Missel, Inga Irle |
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Rok vydání: | 2013 |
Předmět: | |
Zdroj: | BIOspektrum. 19:766-767 |
ISSN: | 1868-6249 0947-0867 |
DOI: | 10.1007/s12268-013-0390-1 |
Popis: | Reliable and sensitive single target copy detection, as well as selection of reliable reference genes for precise gene expression analysis, are still challenges for real-time PCR users. We introduce a system that enables robust and highly sensitive probe-based real-time PCR, provides a novel, antibody-mediated, hot-start mechanism for highly specific target detection, and allows flexible combinations of high and low abundant target and reference genes for accurate gene expression results. |
Databáze: | OpenAIRE |
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