Akkurate Detektion seltener Gene und flexible Auswahl der Referenzgene

Autor: Thorsten Traeger, Andreas Missel, Inga Irle
Rok vydání: 2013
Předmět:
Zdroj: BIOspektrum. 19:766-767
ISSN: 1868-6249
0947-0867
DOI: 10.1007/s12268-013-0390-1
Popis: Reliable and sensitive single target copy detection, as well as selection of reliable reference genes for precise gene expression analysis, are still challenges for real-time PCR users. We introduce a system that enables robust and highly sensitive probe-based real-time PCR, provides a novel, antibody-mediated, hot-start mechanism for highly specific target detection, and allows flexible combinations of high and low abundant target and reference genes for accurate gene expression results.
Databáze: OpenAIRE