RNA-free DNA Extraction Protocol from PinusTissues for Molecular Biology or HPCE/HPLC Analyses

Autor:	Luis Valledor, Rodrigo Hasbún, Roberto Rodríguez, Ma Jesús Cañal
Rok vydání:	2009
Předmět:	Protocol (science) fungi Extraction (chemistry) RNA Plant Science Biology High-performance liquid chromatography DNA extraction Molecular biology In vitro chemistry.chemical_compound chemistry Callus Agronomy and Crop Science DNA Biotechnology
Zdroj:	Journal of Plant Biochemistry and Biotechnology. 18:229-232
ISSN:	0974-1275 0971-7811
DOI:	10.1007/bf03263325
Popis:	A simple method for extracting DNA from various in vitro or ex vitro Pinus tissues is described. Good yield and high quality RNA-free DNA ready to use in molecular biology assays or analytical analysis was obtained. DNA quality was measured analyzing absorption spectra between 200 and 300 nm, A260/280 ratio and HPCE. This protocol was tested with no modification in a wide range of Pinus tissues from recalcitrant in vitro callus to mature field scions improving the results obtained with previous protocols. As the protocol is simple, almost universal and inexpensive it may be used for routine isolation from Pinus tissues. An isolation troubleshooting table was included to facilitate the setting up of reported protocol to other plant species.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::aa5cc864a0419ff7dc1433df1869223f https://doi.org/10.1007/bf03263325 Zobrazit plný text záznamu Full text from SpringerLink