MRI-Based and Histologically Verified 3D Modeling of Spatial Distribution of Intra-Arterially Transplanted Cells in Rat Brain

Autor:	P. A. Mel’nikov, Leonid V. Gubsky, V. A. Revkova, I. L. Gubskiy, Konstantin N. Yarygin, K. K. Sukhinich, Vladimir P. Chekhonin, D. D. Namestnikova
Rok vydání:	2021
Předmět:	Pathology medicine.medical_specialty Therapeutic action Microscopic level Data interpretation General Medicine Intravital Imaging Biology Rat brain General Biochemistry Genetics and Molecular Biology medicine Stem cell Superparamagnetic iron oxide Histological examination
Zdroj:	Bulletin of Experimental Biology and Medicine. 171:517-522
ISSN:	1573-8221 0007-4888
Popis:	Visualization of transplanted stem cells in the brain is an important issue in the study of the mechanisms of their therapeutic action. MRI allowing visualization of single transplanted cells previously labeled with superparamagnetic iron oxide particles is among the most informative methods of non-invasive intravital imaging. Verification of MRI data using pathomorphological examination at the microscopic level helps to avoid errors in data interpretation. However, making serial sections of the whole brain and searching for transplanted cells under the microscope is laborious and time-consuming. We have developed a method for 3D modeling of the distribution of transplanted cells in the brain allowing navigating through various brain structures and identifying the areas of accumulation of transplanted cells, which significantly increases the efficiency and reduces the time of histological examination.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::aa549e8897257fea7ee8dd44efa4d962 https://doi.org/10.1007/s10517-021-05263-8 Zobrazit plný text záznamu Full text from SpringerLink