Bioconversion of Cellulo-Starch Waste from Cassava Starch Industries for Ethanol Production: Pretreatment Techniques and Improved Enzyme Systems
Autor: | M.P. Divya Nair, M.S. Sajeev, J.T. Sheriff, G. Padmaja |
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Rok vydání: | 2012 |
Předmět: | |
Zdroj: | Industrial Biotechnology. 8:300-308 |
ISSN: | 1931-8421 1550-9087 |
Popis: | Cassava starch factory residue (CSFR) is a cellulo-starch byproduct of the starch industries in India that contains approximately 56–60% unextracted starch. The potential of this low-cost biowaste as a raw material for ethanol production was investigated using a combination of pretreatment techniques and improved enzyme systems. Hydrothermal pretreatment for 30 min significantly enhanced the biodegradation of CSFR and the possible loosening of the cellulose/hemicellulose matrix, evidenced through ultrastructural studies. Binary enzyme action using Accellerase™ 1000 (cellulolytic enzyme complex) and Stargen™ 001 (granular starch-hydrolyzing enzyme) was advantageous due to ethanol yield obtained with a shorter processing time (72 h). Non-ionic surfactant such as Tween 20® significantly enhanced the ethanol yield by possible scavenging of lignin, preventing nonproductive binding to cellulase. Scanning electron microscopic studies on pretreated CSFR and the residue left after the saccharification/fer... |
Databáze: | OpenAIRE |
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