Development of a long noncoding RNA BC032469-dependent gold nanoparticle molecular beacon for the detection of gastric cancer cells

Autor:	En Liu, Yu Huang, Yu Feng Xiao, Shuo Zeng, Xiao Yan Zhao, Zhuo Yang, Shi Ming Yang, Su Min Wang
Rok vydání:	2021
Předmět:	Chemistry Oligonucleotide Biomedical Engineering Medicine (miscellaneous) Bioengineering Development Fluorescence Long non-coding RNA In vitro In vivo Molecular beacon Cancer cell Biophysics General Materials Science Intracellular
Zdroj:	Nanomedicine. 16:2255-2267
ISSN:	1748-6963 1743-5889
Popis:	Aim: Long noncoding RNA (lncRNA) BC032469-dependent gold nanoparticle molecular beacons (AuNP-MB) were constructed for the detection of gastric cancer cells. Materials & methods: The AuNP-MBs were prepared according to well-established procedures based on the Au–S interaction between the gold lattice and thiol functionalized oligonucleotides. More importantly, the stability and targeting ability of AuNP-MB were verified by a series of in vitro and in vivo experiments. Results: The lncRNA-dependent probes were successfully utilized for AuNP-MB-based intracellular imaging, with fluorescence effectively emitted in GC cells, but not in normal cells. Notably, such fluorescent emission was positively correlated with lncRNA BC032469 expression. Conclusion: The authors developed an effective fluorescent imaging probe for the recognition of gastric cancer cells.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::aa337f0fbe89b4e0f0554e2f05a18254 https://doi.org/10.2217/nnm-2021-0249 Zobrazit plný text záznamu