EFFECT OF LOW INTENSITY LASER THERAPY (WAVELENGTH 1265 NM) ON THE LEVEL OF ACTIVE OXYGEN AND LIPID PEROXIDATION-ANTIOXIDANT SYSTEM IN CHO-K1 AND A875 CELLS
| Autor: | I I Antoneeva, Evgenii Beloborodov, D.A. Stolyarov, E. S. Pogodina, L.V. Poludnyakova, A.K. Gil'mutdinova, Yu. V. Saenko, Anna Khokhlova, A.Yu. Fedotova |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Antioxidant medicine.medical_treatment Lipid peroxidation Active oxygen 030207 dermatology & venereal diseases 03 medical and health sciences chemistry.chemical_compound Wavelength 030104 developmental biology 0302 clinical medicine chemistry Biophysics medicine Low-intensity laser |
| Zdroj: | Ulyanovsk Medico-biological Journal. :122-132 |
| ISSN: | 2227-1848 |
| DOI: | 10.34014/2227-1848-2020-4-122-132 |
| Popis: | Near-infrared laser therapy has a significantly greater ability to penetrate living tissues compared to visible range optical radiation and often has the opposite effect on normal and malignant cells, thus being a promising tool for superficial neoplasm therapy. However, the mechanisms underlying the interaction between laser and biological structures can be of a different nature due to the chosen wavelength, power, and other radiation parameters. The aim of the paper is to study the efficacy of laser radiation with a wavelength of 1265 nm (9.45–18.9 J/cm2) on the generation of reactive oxygen species (ROS), the enzyme level of the antioxidant defence (AOD) and lipid peroxidation (LPO) in normal and cancer cells of Chinese hamster ovary epithelial cell line CHO-K1 and human melanoma A875. Materials and Methods. The experiment was carried out on cell lines CHO-K1 and A875, which were submitted to laser irradiation (wavelength 1265 nm, power 4.2–100 mW) for 30–60 min. Fluorescent staining with DCFH-DA was used to study intracellular ROS concentration; ImageJ software was used for image processing. The level of LPO products and the activity of AOD enzymes were determined by biochemical methods. Statistical processing of the results was carried out using the Mann–Whitney U-test (p |
| Databáze: | OpenAIRE |
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