| Autor: |
Arun P. Wiita, Bryant Dm, Margaret Guo, Guanqing Ou, FuiBoon Kai, Wei Guo, Viasnoff, Sophie Dumont, Dorit Hanein, Ravi Radhakrishnan, Yisu Han, Alexandra F. Long, Hai Huang, Andrew M. Leidal, Valerie M. Weaver, Richard W. Tourdot, Niels Volkmann, Guido Gaietta, Mark F. Swift, Jason J. Northey, Connor Stashko |
| Rok vydání: |
2021 |
| Předmět: |
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| DOI: |
10.1101/2021.07.14.452329 |
| Popis: |
Primary tissue organoids and cell spheroids recapitulate tissue physiology with remarkable fidelity. We investigated how engagement with a three dimensional laminin-rich extracellular matrix supports the polarized, stress resilient spheroid phenotype of mammary epithelial cells. Cells within a three dimensional laminin-rich extracellular matrix decreased and redistributed the actin crosslinker filamin to reduce their cortical actin tension. Cells with low cortical actin tension had increased plasma membrane protrusions that promoted negative plasma membrane curvature and fostered protein associations with the plasma membrane, consistent with efficient protein secretion. By contrast, cells engaging a laminin-rich extracellular matrix in two dimensions had high filamin-dependent cortical actin tension, exhibited compromised endoplasmic reticulum function including increased expression of PKR-like Endoplasmic Reticulum Kinase signaling effectors, and had compromised protein secretion. Cells with low filamin-mediated cortical actin tension and reduced endoplasmic reticulum stress response signaling secreted, and assembled, a polarized endogenous basement membrane and survived better, and their spheroids were more resistant to exogenous stress. The findings implicate filamin-dependent cortical actin tension in endoplasmic reticulum function and highlight a role for mechanics in organoid homeostasis. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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