Isolation and Characterization of Novel Microsatellite Markers in Chayote [Sechium edule (Jacq.) Sw.]

Autor:	Moisés Cortés-Cruz, Kazuo N. Watanabe, Blanca Amalia Amaro González, Jorge Cadena Íñiguez, Ryoko Machida-Hirano, Kazuto Shirata
Rok vydání:	2015
Předmět:	Genetics Genetic diversity biology Microsatellite Locus (genetics) General Medicine Sechium Allele biology.organism_classification
Zdroj:	American Journal of Plant Sciences. :2033-2041
ISSN:	2158-2750 2158-2742
DOI:	10.4236/ajps.2015.613203
Popis:	Chayote [Sechium edule (Jacq.) Sw.] is an economically important species in Latin America; however, there are very few reports available regarding its genetic diversity. Out of 11 microsatellite markers isolated, 10 loci provided 1 to 7 alleles per locus in a set of Mexican chayote accessions. Observed and expected heterozygosities for each locus ranged from 0.00 to 0.85 and 0.00 to 0.73, respectively. The overall genetic diversity detected by microsatellites was compared with that detected by P450-based analogue markers, a genome-wide dominant marker. Genetic diversity values obtained by the newly designed microsatellite markers were almost equal to the value estimated by PBA markers, but genetic distances calculated by both marker systems were not significantly correlated. Additional microsatellite markers, which could detect more polymorphisms, may be necessary to analyze the genetic diversity and structure of Mexican chayote collections.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::aa1728085af843d534e4350840a3ea25 https://doi.org/10.4236/ajps.2015.613203 Zobrazit plný text záznamu