Combination of micro-dialysis and infrared spectroscopy: a multianalyte assay for accurate biofluid analysis and patient monitoring
| Autor: | H. Michael Heise, Sven Delbeck, Janpeter Budde, Thorsten Vahlsing, Dieter F. Ihrig |
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| Rok vydání: | 2016 |
| Předmět: |
Blood glucose monitoring
Chromatography medicine.diagnostic_test Remote patient monitoring Bicarbonate 010401 analytical chemistry Infrared spectroscopy 030209 endocrinology & metabolism 01 natural sciences 0104 chemical sciences 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine chemistry In vivo medicine Mannitol Dialysis (biochemistry) Quantitative analysis (chemistry) medicine.drug |
| Zdroj: | Biomedical Vibrational Spectroscopy 2016: Advances in Research and Industry. |
| ISSN: | 0277-786X |
| Popis: | Micro-dialysis can be used for continuously harvesting body fluids, while a multi-component analysis of the dialysates by infrared spectrometry offers splendid opportunities for monitoring substrates and metabolites such as glucose, lactate and others small enough to penetrate the semi-permeable dialysis membranes. However, a drawback of this process are variable recovery rates, which can be observed especially for subcutaneously implanted catheters in human subjects. Isotonic perfusates were investigated with acetate and mannitol as recovery markers for the dialysis of human serum at 37°C to mimic in vivo patient monitoring. The latter non-ionic substance has been suggested for application when other ionic substances such as bicarbonate or pH are also to be determined. Simultaneously for acetate and mannitol, the depletion of the marker substances from the perfusates using different micro-dialysis devices was investigated under various flow-rates. Relationships between relative dialysate marker concentrations and glucose recovery rates were determined based on multivariate calibrations. For quantification, classical least squares with reference spectra for modelling the serum dialysates was used, rendering a basis for reliable blood glucose and lactate measurements. |
| Databáze: | OpenAIRE |
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