THE DEVELOPMENT OF CONDUCTOMETRIC BIOSENSOR FOR DETERMINATION OF URIC ACID CONCENTRATION IN HUMAN SERUM USING SCREEN PRINTED CARBON ELECTRODE (SPCE) – NATA DE COCO
| Autor: | Abdi Naufal Ramadhan, Noerma Juli Azhari, Luluil Maknun |
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| Rok vydání: | 2016 |
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| Zdroj: | ALCHEMY Jurnal Penelitian Kimia. 11:192 |
| ISSN: | 2443-4183 1412-4092 |
| DOI: | 10.20961/alchemy.11.2.724.192-199 |
| Popis: | The control of uric acid is important. A high level of uric acid can cause gout disease. Therefore a simple, fast, and accurate method for uric acid determination is required. In this research a conductometric biosensor has been developed by SPCE – Nata de coco for uric acid determination. The prepared biosensor was optimized to get a good performance of biosensor and it is applicable for human serum samples. The optimized variables were enzyme concentration, membrane thickness and pH solution. The various enzyme concentration were 6 μg/mL; 12 μg/mL; 18μg/mL; 24 μg/mL. The various membrane thickness were 5 μm; 10 μm; 15 μm. Meanwhile, the various pH solution were 7; 7.5; 8; 8.5; 9. The optimum enzime concentration was 18 µg/mL with the membrane thickness and pH were 5 µm and 8, respectively. The prepared biosensor can determine the uric acid concentration at range of 0 – 1.2 ppm with the sensitivity of 7.74 µS/ppm and the limit detection is 0 ppm. The biosensor was applied to uric acid detection in human serum with accuracy of 95 %. |
| Databáze: | OpenAIRE |
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