Intrinsic Bio-Enhancer Entities of Fagonia cretica for Synthesis of Silver Nanoparticles Involves Anti-Urease, Anti-Oxidant and Anti-Tyosinase Activity
| Autor: | Ayesha Zafar, Shahbaz Gul Hassan, Aqsa Yousaf, Muhammad Ali, Tuba Tariq, Muhammad Abdullah, Faryal Bukhari, Muhammad Akram, Murtaza Hasan, Yasmeen Manzoor, Snober Mona Bukhary, Syeda Sadaf Zehra, Asma Saeed |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
chemistry.chemical_classification Antioxidant Urease biology Chemistry medicine.medical_treatment Nanoparticle Fraction (chemistry) Biological activity General Medicine 030105 genetics & heredity biology.organism_classification Silver nanoparticle 03 medical and health sciences 030104 developmental biology Enzyme Fagonia cretica biology.protein medicine Nuclear chemistry |
| Zdroj: | Advances in Bioscience and Biotechnology. 10:455-468 |
| ISSN: | 2156-8502 2156-8456 |
| DOI: | 10.4236/abb.2019.1012032 |
| Popis: | Objective: Evaluating the bio-reducing activity and various potential, annotates the biological entitties of Fagonia cretica. Method: By fractionating the crude extract with different ethanol-water fraction mixture. This substantiates the reduction potential for the biological synthesis of AgNPs, its antioxidant, anti-urease and anti-Tyrosinase activity. Results: UV-visible spectra confirm the bio-reducing potential for Ag NPs with the optimized 50% ethanol-water fraction mixture. Transmission electron microscopy (TEM) confirmed the round shaped AgNPs with an average size of 16 nm in optimized 50% fraction mixture. Further evaluating Ethanol-water fraction with 90% and 70% of plant extract showed more potentials for Anti-Tyrosinase and Anti-urease activity. Moreover, diluted 30% fraction exhibits two folds higher Anti-oxidant and Anti urease activity. Conclusion: Overall, this work showed that 50% Ethanol-water fraction has potentially active molecules (among all fractions) primarily involved in bio-reducing potential of Ag NPs and enzymatic assay while 90% mixture proved to be least active. This study suggested electing the optimum mixture fraction of 50% ethanol-water in order to screen the bioactive compounds and to inculcate their activity status for clinical trials. |
| Databáze: | OpenAIRE |
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