Hemolyzed Blood Elicits a Calcium Antagonist and High CO2Reversible Constriction via Elevation of [Ca2+]iin Isolated Cerebral Arteries
| Autor: | Peter Cseplo, Ivan Ivic, Andras Buki, Akos Koller, Orsolya Torok, Attila Tóth, Zoltan Vamos |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
medicine.medical_specialty
Subarachnoid hemorrhage Vasomotor business.industry Cerebral arteries Vasodilation 030204 cardiovascular system & hematology medicine.disease 03 medical and health sciences Red blood cell 0302 clinical medicine medicine.anatomical_structure Endocrinology Internal medicine medicine.artery Anesthesia Middle cerebral artery cardiovascular system medicine Basilar artery Neurology (clinical) medicine.symptom business 030217 neurology & neurosurgery Vasoconstriction |
| Zdroj: | Journal of Neurotrauma. 34:529-534 |
| ISSN: | 1557-9042 0897-7151 |
| Popis: | During acute subarachnoid hemorrhage, blood is hemolyzed, which is followed by a significant cerebrovascular spasm resulting in a serious clinical condition. Interestingly, however, the direct vasomotor effect of perivascular hemolyzed blood (HB) has not yet been characterized, preventing the assessment of contribution of vasoconstrictor mechanisms deriving from brain tissue and/or blood and development of possible treatments. We hypothesized that perivascular HB reduces the diameter of the cerebral arteries (i.e., basilar artery [BA]; middle cerebral artery [MCA]) by elevating vascular tissue [Ca2+]i level. Vasomotor responses were measured by videomicroscopy and intracellular Ca2+ by the Fura2-AM ratiometric method. Adding HB to the vessel chamber reduced the diameter significantly (BA: from 264 ± 7 to 164 ± 11 μm; MCA: from 185 ± 15 to 155 ± 14 μm), which was reversed to control level by wash-out of HB. Potassium chloride (KCl), HB, serum, hemolyzed red blood cell (RBC), plasma, and platelet s... |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|