| Popis: |
Modern methods for quantifying signaling bias at GPCRs rely on using a single β-arrestin isoform. However, it is increasingly appreciated that the two β-arrestin isoforms have unique roles, requiring the ability to assess β-arrestin isoform preference. Herein, we present ClickArr, a live-cell assay that simultaneously reports recruitment of both β-arrestin isoforms as they compete for interaction with GPCRs. We demonstrate that an agonist can have β-arrestin isoform bias, potentially opening up a new dimension for drug development. |
