Significance of previtamin D chromatographic resolution in the accurate determination of vitamin D3 by HPLC‒UV
| Autor: | Brendon D. Gill, David C. Woollard, Harvey E. Indyk |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Vitamin
0303 health sciences Chromatography Resolution (mass spectrometry) 030309 nutrition & dietetics Chemistry 010401 analytical chemistry 01 natural sciences High-performance liquid chromatography 0104 chemical sciences 03 medical and health sciences chemistry.chemical_compound Vitamin D and neurology lipids (amino acids peptides and proteins) Uv detection Food Science |
| Zdroj: | Journal of Food Composition and Analysis. 79:1-4 |
| ISSN: | 0889-1575 |
| DOI: | 10.1016/j.jfca.2019.02.010 |
| Popis: | Conventional methods using HPLC with UV detection have used vitamin D2 as an internal standard with the expectation that this fully compensates for the heat-dependent equilibrium of vitamin D3 with its previtamin. Previtamin D has a different spectral absorptivity from vitamin D and may be present in different proportions in samples and standards. Therefore, vitamin D2 and vitamin D3 and their previtamin forms must be chromatographically resolved to achieve accurate quantitation of total vitamin D. This study identified four chromatographic columns (ACE C18, ACE C18 AR, Vydac 201 TP C18 and Polaris C18-Ether) with adequate selectivity that should be applied for food testing and further confirmed that both parent vitamins isomerise at the same rate under thermal conditions. |
| Databáze: | OpenAIRE |
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