Identification of alfonsino and related fish species belonging to the genus Beryx with mitochondrial 16S rRNA gene and its application on their pelagic eggs

Autor:	Shugo Watabe, Shigeharu Kinoshita, Keijiro Sezaki, Isamu Mitani, Seiji Akimoto
Rok vydání:	2002
Předmět:	Genetics Mitochondrial DNA biology Nucleic acid sequence Zoology Aquatic Science biology.organism_classification 16S ribosomal RNA law.invention Beryx law Genus Alfonsino Gene Polymerase chain reaction
Zdroj:	Fisheries Science. 68:1242-1249
ISSN:	1444-2906 0919-9268
Popis:	The mitochondrial 16S rRNA gene was amplified by polymerase chain reaction usingtotal DNAas a template from muscle tissues of alfonsino and related fish species of alfonsino belong-ing to the genus Beryx , B.splendens , B.mollis and B.decadactylus . While the sequence of the 16SrRNA gene consisted of 492 nucleotides irrespective of the three species, three, nine and eight inter-specific variations were shown between B.splendens and B.mollis , between B.splendens and B.decadactylus , and between B.mollis and B.decadactylus , respectively. The pelagic eggs, which werecollected off Izu Islands and showed morphological characteristics typical to the genus Beryx wereall identified as those of B.splendens from the nucleotide sequence of the mitochondrial 16S rRNA gene. KEY WORDS: 16S rRNA , alfonsino, Beryx , mitochondrial DNA, pelagic eggs, species identification. *Corresponding author: Tel: 81-468-82-2313. Fax: 81-468-81-7903. Email: akimoto@agri.pref.kanagawa.jpReceived 28 December 2001. Accepted 4 June 2002.
Databáze:	OpenAIRE
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