Eliminating and inhibiting hydroxylamine oxidation in DEPMPO spin trapping experiments
| Autor: | Yuan Liu, Bin Liu, Yu Guang Song, Yang Ping Liu, Zhou Nie, Qiu Tian, Ke-Jian Liu |
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| Rok vydání: | 2005 |
| Předmět: |
Nitroxide mediated radical polymerization
Aqueous solution Spin trapping Chemistry Inorganic chemistry chemistry.chemical_element Photochemistry Oxygen Atomic and Molecular Physics and Optics law.invention chemistry.chemical_compound Hydroxylamine Impurity law Chelation Electron paramagnetic resonance |
| Zdroj: | Applied Magnetic Resonance. 29:597-604 |
| ISSN: | 1613-7507 0937-9347 |
| DOI: | 10.1007/bf03166336 |
| Popis: | There exists hydroxylamine impurity in 5-(diethoxyphosphoryl)-5-methyl-l-pyrroline-l-oxide (DEPMPO) despite the purification by column separation and molecular distillation. Slow oxidation of hydroxylamine impurity to nitroxide is caused by a trace amount of transition metal ions in the solution instead by oxygen, and it can be conveniently inhibited upon the addition of diethylene-triaminepentaacetic acid, a transition metal ion chelator. Hydroxylamine impurity in DEPMPO, can also be oxidized to electron spin resonance detectable paramagnetic nitroxide in the presence of mild oxidants, such as K3Fe(CN)6 and CuSO4. The previously recommended procedure for elimination of hydroxylamines was further simplified in the present study. Both hydroxylamine and nitroxide impurities can be removed from the aqueous solution of DEPMPO, by activated charcoal treatment. |
| Databáze: | OpenAIRE |
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