Assessment of a new strategy for selective phenotyping applied to complex traits in Brassica napus
| Autor: | Maria J. Manzanares-Dauleux, Régine Delourme, Claude Domin, C. Jestin, Patrick Vallée |
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| Rok vydání: | 2012 |
| Předmět: |
0106 biological sciences
Genetics 0303 health sciences education.field_of_study Population food and beverages Biology Quantitative trait locus biology.organism_classification 01 natural sciences 03 medical and health sciences Pathosystem Leptosphaeria maculans Family-based QTL mapping Sample size determination Inclusive composite interval mapping Trait education 030304 developmental biology 010606 plant biology & botany |
| Zdroj: | Open Journal of Genetics. :190-201 |
| ISSN: | 2162-4461 2162-4453 |
| DOI: | 10.4236/ojgen.2012.24025 |
| Popis: | The accurate mapping of quantitative trait loci (QTL) depends notably on the number of recombination events occurring in the segregating population. The cost of phenotyping often limits the sample size used in QTL mapping. To get round this problem, we assessed a selective phenotyping method, called qtlRec sampling. In order to improve the accuracy of QTL mapping, a subset of individuals was selected to maximize the number of recombination events at putative QTL positions; the usefulness of this subset was compared to a selected sample built to maximize the recombination rate over the whole genome. We assessed this method on the quantitative oil content trait in Brassica napus. We showed that the qtlRec strategy could allow increasing accuracy (both support interval and position) of QTL location while it maintained a similar power of detection. We then applied this approach to the B. napus—Leptosphaeria maculans pathosystem for which resistance QTL with minor effect were previously identified. This allowed the validation of the QTL in six genomic regions. The qtlRec method is an attractive strategy for validating QTL in multiple year and/or location trials for a trait which requires costly and time-consuming phenotyping. |
| Databáze: | OpenAIRE |
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