Viscosity‐Temperature Dependence on DNA Stretching: Slalom Chromatography Study

Autor:	Alain Xicluna, Yves Claude Guillaume, Jean François Robert, Laurence Nicod, J. Millet, Mireille Thomassin, Eric Peyrin, Tong Thanh Truong
Rok vydání:	2003
Předmět:	chemistry.chemical_classification Quantitative Biology::Biomolecules Chromatography Clinical Biochemistry Analytical chemistry Pharmaceutical Science Polymer Biochemistry High-performance liquid chromatography Analytical Chemistry Viscosity chemistry.chemical_compound chemistry Temperature dependence of liquid viscosity Phase (matter) Molecule Phase velocity DNA
Zdroj:	Journal of Liquid Chromatography & Related Technologies. 26:883-893
ISSN:	1520-572X 1082-6076
DOI:	10.1081/jlc-120018890
Popis:	Slalom chromatography (SC) is a chromatographic procedure for the separation of polymers, which is based on a non equilibrium principle. A novel equation was recently developed to model the retention variation of linear double stranded DNA molecules with the mobile phase velocity under SC. This paper analyzes the effect of mobile phase viscosity and temperature on the constants of this equation and confirms that these two factors play a great role on the DNA fragment stretching.
Databáze:	OpenAIRE
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