Applying Aluminum Oxide Column Chromatography Purify Extracts ofCathuranthus Roseusand Simultaneous Determination of the Indole Alkaloids by HPLC

Autor:	Liqun Rao, Zhonghai Tang
Rok vydání:	2010
Předmět:	Indole test Chromatography Chloroform Indole alkaloid Biochemistry (medical) Clinical Biochemistry Ether complex mixtures Biochemistry High-performance liquid chromatography Analytical Chemistry chemistry.chemical_compound Column chromatography chemistry Electrochemistry Petroleum ether Spectroscopy Vindoline
Zdroj:	Analytical Letters. 43:219-225
ISSN:	1532-236X 0003-2719
DOI:	10.1080/00032710903325773
Popis:	Indole alkaloid of Cathuranthus roseus was purified by applying aluminum oxide column chromatography and an HPLC method was established to determine it. Applying aluminum oxide column chromatography produced a high purified monomer of alkaloids; the extracts of indole alkaloid were then loaded on the neutral aluminum oxide column chromatography and washed with an organic solvent (a mixture of chloroform, ether, and petroleum ether). Effect of purification was the best when the proportion of chloroform, ether, and petroleum ether was 10:10:1. To determine the content of vindoline, vinblastine, and cathuranthine exactly, the mobile phase of HPLC was water (containing 0.005 mol·L−1 ammonium hydrogen phosphate), methanol (containing 0.67% triethylamine), and acetonitrile, the proportion of each was nonlinear.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::a9b4492cdd7473a4741c57e2a513f788 https://doi.org/10.1080/00032710903325773 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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