Is Hemoglobin Antigenic?
| Autor: | Carl L. A. Schmidt, C. B. Bennett |
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| Rok vydání: | 1919 |
| Předmět: | |
| Zdroj: | Journal of Infectious Diseases. 25:207-212 |
| ISSN: | 1537-6613 0022-1899 |
| DOI: | 10.1093/infdis/25.3.207 |
| Popis: | Thus in work reported by Leblanc,1 a precipitin for beef hemoglobin was obtained by immunizing a dog with a preparation obtained by saturating laked, washed, red cells with ammonium sulphate after removal of globulins and stromata. This preparation was by no means pure since no attempt was made to free the hemoglobin from possible contamination by other proteins which may have been carried down by the crystals. Levene1' was unable to produce either a lysin or an agglutinin for red cells by immunization with twice crystallized hemoglobin. Ford and Halsey,3 working with various fractions of the red cell, were unable to demonstrate antibodies?precipitins, lytic or agglutinating substances in rabbits or anaphylaxis in guinea-pigs injected with dog or hen hemoglobin recrystallized 4-5 times from 25*^ alcohol. These authors used special precautions to exclude protein contamination and their results appear to be more reliable than other workers in this field. Schittenhelm and |
| Databáze: | OpenAIRE |
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