| Popis: |
Two cultivars of Phaseolus vulgaris L., flor de mayo FM-RMC, showing resistance to common mosaic virus and to bean rust (Uromyces phaseoli), and FM-C, without such resistance, were used to study the synthesis of lectins during seed development as measured by affinity chromatography of protein fractions. Seeds at different stages of development were sorted out by size from 4–12 mm length, in addition to mature samples. The specific haemagglutinating activity (SHA) of isolated protein fractions from mature seeds, expressed as haemagglutinating units/mg protein, and the theoretical agglutination capacity (TAC). calculated from the SHA and the total protein fraction, were also assessed. The peak lectin level as measured by affinity chromatography was reached at a seed size of 10 mm and decreased thereafter. The lectin content and TAC of all samples of the disease-resistant cultivar were superior to that of the non-resistant variety. |
