Autoantibodies directed against CD43 molecules with an altered glycosylation status on human immunodeficiency virus type 1 (HIV-1)- infected CEM cells are found in all HIV-1+ individuals
| Autor: | J Cottalorda, Jean-Gabriel Fuzibet, Alain Doglio, A Passeron, Jean-Claude Lefebvre, V Giordanengo, M Limouse, L Desroys du Roure |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
chemistry.chemical_classification
Glycosylation Lymphocyte Immunology Autoantibody hemic and immune systems Cell Biology Hematology Biology Biochemistry Virology Virus In vitro chemistry.chemical_compound medicine.anatomical_structure chemistry Antigen immune system diseases hemic and lymphatic diseases medicine biology.protein Antibody Glycoprotein |
| Zdroj: | Blood. 86:2302-2311 |
| ISSN: | 1528-0020 0006-4971 |
| DOI: | 10.1182/blood.v86.6.2302.bloodjournal8662302 |
| Popis: | Autoantibodies to lymphocytes have been detected in sera from human immunodeficiency virus type 1 (HIV-1)-infected individuals, and several autoantigens have been described. Among them, hyposialylated CD43 has been shown to be a target for autoantibodies in up to 47% of HIV+ individuals. However, the corresponding autoantigen (ie, the incompletely sialylated CD43) has not been isolated from blood cells of HIV-1-infected individuals. Recently, we have observed in vitro that HIV-1 productively or latently infected CEM cells (CEMLAI/NP) express CD43 molecules with modified glycosylation (mogly CD43). Using CEMLAI/NP cells, which do not express any structural viral antigen, we show now that all of the tested HIV+ sera from asymptomatic individuals, and up to 86% of those from subjects at the acquired immunodeficiency syndrome stage contain antibodies (mainly IgM and, to a lesser degree, IgG) that recognize the surface of CEMLAI/NP cells, and precipitate mogly CD43 molecules from the cells lysates. Taken together with our previous demonstration of altered glycosylation of CD43 from HIV-1-infected CEM cells in vitro, the constant antimogly CD43 autoimmune response observed from asymptomatic HIV-1+ subjects is likely to illustrate the occurrence of an altered glycosylation in vivo of the major lymphocyte surface CD43 glycoprotein, associated with HIV- 1 infection. |
| Databáze: | OpenAIRE |
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