| Autor: |
JoSé L Feijoo, Karem Noris-Suárez, Gema González, Rodolfo J. González-Paz |
| Rok vydání: |
2020 |
| Předmět: |
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| Zdroj: |
Advanced Science, Engineering and Medicine. 12:576-581 |
| ISSN: |
2164-6627 |
| DOI: |
10.1166/asem.2020.2558 |
| Popis: |
In a previous study of the deposition of nanohydroxyapatite on demineralized cortical bone collagen stimulated by deformation-induced piezoelectricity was obtained in our lab. Thus, the aim of the present study was to characterize the interactions between the self-assembled nanostructure of bone collagen and water in presence and absence of hydroxyapatite (HAP) to elucidate which factors allowed such deposition in just four weeks without the presence of bone cells. It was found that the loss of the mineral phase in bone (HAP) contributes to decrease the thermal stability of the collagen nanostructures. An important increase on the free, bonded and structural water content was found in the bone matrix, directly related to the degree of demineralization reached by the collagen matrix with time. This demonstrates that the collagen nanostructure is able to restructure itself while losing the interactions with the mineral phase, establishing new interactions with the water molecule, orienting the dipoles of the collagen molecule. All these increase the piezoelectric character of the bone matrix and allows the collagen matrix to get prepared for a bone re-mineralization process without bone cells, due to the electrostatic interactions established with the calcium and phosphate ions of the external fluid of the extracellular matrix. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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