Comparison of isocratic and gradient elution reversed-phase behaviour of high-molecular-mass polystyrenes in dichloromethane and acetonitrile

Autor:	Ian Maxwell Russell, Ross Andrew Shalliker, P. E. Kavanagh
Rok vydání:	1994
Předmět:	Chromatography Elution Organic Chemistry Analytical chemistry General Medicine Reversed-phase chromatography Biochemistry Capacity factor Analytical Chemistry Gel permeation chromatography chemistry.chemical_compound chemistry Phase (matter) Solvent effects Acetonitrile Dichloromethane
Zdroj:	Journal of Chromatography A. 679:105-114
ISSN:	0021-9673
DOI:	10.1016/0021-9673(94)80316-1
Popis:	Isocratic and gradient elution of high-molecular-mass polystyrenes were compared in a dichloromethane-acetonitrile mobile phase and C 18 bonded phase on a variety of different pore size silicas. Plots of the capacity factor versus the solvent composition were drawn from which solvent strength parameters, S , were determined for different molecular masses. Linear gradient elution data were also used to estimate S values using a graphical procedure. It was found that S depended on gradient rate and that only gradient rates of less than 2% /min gave constant values of S . Only molecular masses less than 50 000 gave good agreement with isocratic values. Plots of log S against log molecular mass were different for isocratic and gradient determined values of S .
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::a992b5ae5d45f782109e221744f7e9ad https://doi.org/10.1016/0021-9673(94)80316-1 Zobrazit plný text záznamu