Phosphate-Regulated Induction of Intracellular Ribonucleases in Cultured Tomato (Lycopersicon esculentum) Cells

Autor:	Steffen Abel, Konrad Glund, Andreas Löffler, Wolfgang Jost, Jaap J. Beintema
Rok vydání:	1992
Předmět:	chemistry.chemical_classification biology Physiology RNase P food and beverages Plant Science Vacuole Phosphate chemistry.chemical_compound Enzyme Isoelectric point chemistry Biochemistry Genetics biology.protein Extracellular Ribonuclease Intracellular
Zdroj:	Plant Physiology. 98:1472-1478
ISSN:	1532-2548 0032-0889
DOI:	10.1104/pp.98.4.1472
Popis:	Four intracellular RNases were found to be induced in cultured tomato (Lycopersicon esculentum) cells upon phosphate starvation. Localization studies revealed three (RNases LV 1-3) in the vacuoles and one (RNase LX) outside these organelles. All of these RNases were purified to homogeneity and were shown to be type I RNases on the basis of type of splitting, substrate, and base specificity at the cleavage site, molecular weight, isoelectric point, and pH optimum. Moreover, RNase LV 3 was shown by fingerprinting of tryptic digests on reversed-phase high-performance liquid chromatography and sequencing the N terminus and two tryptic peptides to be structurally very similar to a recently characterized extracellular RNase LE which is also phosphate regulated (Nurnberger et al. [1990] Plant Physiol 92: 970-976; Jost et al. [1991] Eur J Biochem 198: 1-6). Expression of the four intracellular RNases is induced by depleting the cells of phosphate and repressed by adding phosphate. Our studies indicate that higher plants, in addition to secreting enzymes for scavanging phosphate under starvation conditions, also induce intracellularly emergency rescue systems.
Databáze:	OpenAIRE
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