Phloroglucinol functions as an intracellular and intercellular chemical messenger influencing gene expression inPseudomonas protegens
| Autor: | Alex Buchanan, Oliver B. Vining, Joyce E. Loper, Jennifer C. Clifford, Teresa A. Kidarsa, Jeff H. Chang, Kerry L. McPhail |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Regulation of gene expression biology Phloroglucinol biology.organism_classification Microbiology Transcriptome 03 medical and health sciences Pseudomonas protegens chemistry.chemical_compound 030104 developmental biology Biochemistry chemistry Transcription (biology) Gene expression Autoinducer Gene Ecology Evolution Behavior and Systematics |
| Zdroj: | Environmental Microbiology. 18:3296-3308 |
| ISSN: | 1462-2912 |
| DOI: | 10.1111/1462-2920.13043 |
| Popis: | Bacteria can be both highly communicative and highly competitive in natural habitats and antibiotics are thought to play a role in both of these processes. The soil bacterium Pseudomonas protegens Pf-5 produces a spectrum of antibiotics, two of which, pyoluteorin and 2,4-diacetylphloroglucinol (DAPG), function in intracellular and intercellular communication, both as autoinducers of their own production. Here, we demonstrate that phloroglucinol, an intermediate in DAPG biosynthesis, can serve as an intercellular signal influencing the expression of pyoluteorin biosynthesis genes, the production of pyoluteorin, and inhibition of Pythium ultimum, a phytopathogenic oomycete sensitive to pyoluteorin. Through analysis of RNAseq data sets, we show that phloroglucinol had broad effects on the transcriptome of Pf-5, significantly altering the transcription of more than two hundred genes. The effects of nanomolar versus micromolar concentrations of phloroglucinol differed both quantitatively and qualitatively, influencing the expression of distinct sets of genes or having opposite effects on transcript abundance of certain genes. Therefore, our results support the concept of hormesis, a phenomenon associated with signalling molecules that elicit distinct responses at different concentrations. Phloroglucinol is the first example of an intermediate of antibiotic biosynthesis that functions as a chemical messenger influencing gene expression in P. protegens. |
| Databáze: | OpenAIRE |
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