Characterization of a new Pseudomonas isolate, capable of accumulating polyesters of medium chain length 3-hydroxyalkanoic acids

Autor: Ewald B. M. Denner, Gerhard Schroll, Hans-Jürgen Busse, Sabine Rölleke, Werner Lubitz
Rok vydání: 1996
Předmět:
Zdroj: Journal of Biotechnology. 47:53-63
ISSN: 0168-1656
Popis: In a screening program for bacterial strains which produce interesting compositions of polyhydroxyalkanoates (PHAs) the strain PHA1 was isolated displaying an unusual PHA pattern. The presence of ubiquinone with nine isoprenoid units in the side chain (Q-9), putrescine and spermidine as the dominating compounds in the polyamine pattern, the characteristic fatty acid profile as well as partial 16S rDNA analysis clearly indicated, that the isolate is a member of the genus Pseudomonas and closely related to Pseudomonas fluorescens . The strain PHA1 and Pseudomonas fluorescens bv. IV DSM 50415 were investigated for PHA accumulation capabilities. In both strains, alkanoates which corresponded to the carbon chain length (C6–C10) of the fatty acids added as substrate dominated in the PHA patterns and the carbon chain length was reduced by two C-atoms when undecanoate or dodecanoate was fed. Most of the other polymers produced consisted mainly of decanoate or dodecanoate. When the complex medium was supplemented with butyric acid the incorporation of 3-OH butyric acid into the polymer was observed. This resulted in the production of PHA blends or possibly even in the production of mixed copolymers with a significant amount of 3-OH butyric acid in addition to medium chain length PHAs.
Databáze: OpenAIRE
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