Measurement of Resting and Stress-elevated Serum Cortisol in Rainbow Trout Oncorhynchus mykiss in Experimental Net-pens

Autor:	Michael T. Collins, Clyde H. Amundson, Jeffrey A. Malison, Terrence B. Kayes, Myron J. Kebus, Mark S. Brownfield
Rok vydání:	1992
Předmět:	endocrine system medicine.diagnostic_test Aquatic Science Biology Elevated serum Fishery Animal science Immunoassay Anesthetic medicine Fish Rainbow trout Acute stress Agronomy and Crop Science Cortisol level Serum cortisol medicine.drug
Zdroj:	Journal of the World Aquaculture Society. 23:83-88
ISSN:	1749-7345 0893-8849
Popis:	A commercially available heterogeneous, solid-phase tube enzyme-linked immunoassay (ELISA) was modified and validated for the measurement of serum cortisol in rainbow trout Oncorhynchus mykiss. The assay is accurate and precise. Resting and stress-elevated serum cortisol concentrations were measured in rainbow trout with a sensitivity of 1.5 ng/ml. Fish held in net-pens at a density of 0.4 kg/m3/cm had a resting cortisol level of 16.5 ± 3.8 ng/ml (mean ± SE). At 3 h postdisturbance, serum cortisol levels were not affected by the removal of fish from adjacent net-pens with dip nets or by the use of 200 mg/L tricaine methanesulfonate (MS-222) as an anesthetic for obtaining samples. However, an acute stress (60 s removal from water) elevated serum cortisol levels to 73.7 ± 9.4 ng/ml.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::a96764a11858a114ba7480a6bd30195d https://doi.org/10.1111/j.1749-7345.1992.tb00754.x Zobrazit plný text záznamu