Determination of Retinyl Palmitate (Vitamin A) in Fortified Fluid Milk by Liquid Chromatography: Collaborative Study
| Autor: | Douglas A. Hite |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Pharmacology
Vitamin Chromatography food.ingredient Retinol food and beverages Repeatability Retinyl acetate food.food Analytical Chemistry Matrix (chemical analysis) chemistry.chemical_compound food chemistry Retinyl palmitate Skimmed milk Chocolate milk Environmental Chemistry Agronomy and Crop Science Food Science |
| Zdroj: | Journal of AOAC INTERNATIONAL. 86:375-385 |
| ISSN: | 1944-7922 1060-3271 |
| DOI: | 10.1093/jaoac/86.2.375 |
| Popis: | A liquid chromatographic (LC) method was developed for fast and simple measurement of retinyl palmitate (vitamin A) in fortified milk. Retinyl acetate internal standard was added to a test portion of milk followed by extraction into hexane. The hexane extract was analyzed by LC using a normal-phase silica gel column equilibrated with mobile phase (conditioned hexane–isopropanol, 99.85 + 0.15, v/v) about 1 h before injections. The retinyl palmitate concentration was calculated by using a relative response factor determined with calibration standards. In the collaborative study, 11 laboratories analyzed 13 pairs of fluid milk materials in blind duplicate. Twelve of the materials were composed of skim milk ( |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|