Accumulation of AGEs and VEGF in Eyes of SDT Rats
| Autor: | Nozomi Kinoshita, Akihiro Kakehashi, Hiroko Yamagami, Kana Hashimoto, Masanobu Kawakami, San-e Ishikawa, Fumihiko Toyoda, Chiho Kanbara, Hiroyuki Tamemoto, Yasunori Kanazawa, Yoh Dobashi |
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| Rok vydání: | 2011 |
| Předmět: |
medicine.medical_specialty
genetic structures biology business.industry Endocrinology Diabetes and Metabolism VEGF receptors Diabetic retinopathy medicine.disease eye diseases Pathogenesis Vascular endothelial growth factor chemistry.chemical_compound Endocrinology chemistry Glycation Internal medicine medicine biology.protein Immunohistochemistry sense organs Pentosidine business Immunostaining |
| Zdroj: | The Open Diabetes Journal. 4:41-44 |
| ISSN: | 1876-5246 |
| DOI: | 10.2174/1876524601104010041 |
| Popis: | Background/Aims: The Spontaneously Diabetic Torii (SDT) rat develops advanced diabetic retinopathy (DR). The aim of this study was to identify advanced glycation end products (AGEs) related to vascular endothelial growth factor (VEGF) expression, a cause of DR in SDT rats. Methods: One eye was obtained from six SDT rats (blood glucose, >250 mg/dl) and 10 nondiabetic normal Sprague- Dawley (SD) rats and prepared for immunohistochemical study of VEGF and AGEs (pyrraline, pentosidine, carboxy methyl lysine (CML)). Immunostaining was described as minimal, moderate, and severe. Results: In diabetic rats, for CML, five eyes had severe and one moderate immunostaining. For pyrraline, one eye had moderate and five eyes minimal immunostaining. For pentosidine, one eye had moderate and five eyes minimal immunostaining. For VEGF, three eyes each had moderate and severe immunostaining. In nondiabetic rats, for CML one eye had minimal, seven had moderate, and two had severe immunostaining. For pyrraline, four eyes had moderate and six eyes minimal immunostaining. For pentosidine, 10 eyes had minimal immunostaining. For VEGF, one eye had moderate and nine had minimal immunostaining. The prevalence rates of CML and VEGF were significantly (P |
| Databáze: | OpenAIRE |
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